selective and differential media lab report conclusion

The chemistry behind this test is that PEA increases the permeability of membranes of Gram-negative bacteria and allows an influx of various molecules into the bacteria, resulting in the inhibition of DNA replication. conducive for their growth. regions that a yellow and a pink region separated. You will find more specific procedures for each biochemical test on the BLUISH / GREEN bacterial colonies = RISE in pH of media = NO fermentation of sugars but breakdown of proteins = alkaline products made State the usefulness of Columbia CNA agar and MacConkey agar. WebDifferential Media. E. coli is a gram-negative, rod-shaped, facultative anaerobic Objectives. We have included the basic procedure for doing each biochemical test in the table below. In Lab 4 we will use this technique as part of the plate count method of enumerating bacteria. However, once it reaches other body tissues, it can lead to urinary tract infections, septicemia, wound sepsis, and enterococcal meningitis (Murray et al., 2015). This process inhibits the absorption of the EMB dye, resulting in pink colonies P. aeruginosa. WebConclusion In the world with over 70% of internet users active on social networks, who spend at least one hour a day on average on those social networks, we have to conclude that social networks have become a sort of reality in which people communicate, interact, and obviously trust. Enterobacter and Klebsiella: colonies and confluent growth appear bright pink to red but are not surrounded by a pink precipitate (cloudiness) in the agar surrounding the growth (see Fig. Using the streak plate method of isolation, obtain isolated colonies from a mixture of microorganisms. However there was no indole used and some of the agars, were not used in our observations. formation of two zones, one is yellow and the other pink. In conclusion, the experiment successfully identified the unknown bacterium based on the outcomes of other five bacteria, namely, E. coli, E. aerogenes, P. aeruginosa, S. aureus, E. faecalis. Preparation of Medium: Gancyclovir is an exception to the rule as it is used to specifically kill cells that carry its respective marker, the Herpes simplex virus thymidine kinase (HSV TK). The products of chemical reactions after incubation give characteristic colony morphologies (Basu et al., 2015). Staphylococcus aureus ferments mannitol, an acid byproduct is produced which subsequently Add 3.25 grams of the TSB powder to this flask and allow it to dissolve (will happen quickly). A sterile, bent-glass rod is then used to spread the bacteria evenly over the entire agar surface (see Fig. Record your observations and conclusions. 4 and Fig. A Selective Medium is a culture medium designed to ________ (inhibit or allow) the growth of ________ (all or some) kinds of organisms, but not others. It is, therefore, said to be selective for Gram-positive organisms, and would be useful in separating a mixture of Gram-positive and Gram-negative bacteria. How are "superbugs" different from a wild type organism? food microbiology, 149 (3), 194-208. Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS). The LibreTexts libraries arePowered by NICE CXone Expertand are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the California State University Affordable Learning Solutions Program, and Merlot. WebSelective media contains substances that. 2. These are used only for enteric bacteria which are found in the. Selective culture medium for the enumeration of Lactobacillus plantarum in the presence of Eosin Y and Methylene Blue dyes inhibit the growth of Gram positive bacteria (only Gram negative bacteria will grow well), Differential After incubation, the area at the beginning of the streak pattern will show confluent growth while the area near the end of the pattern should show discrete colonies (see Fig. Media lacking an amino acid such as proline in conjunction with E. coli unable to synthesize it were commonly used by geneticists before the emergence of genomics to map bacterial chromosomes. Microorganisms exhibit distinct colonial growth characteristics in different culture conditions, allowing for strain-level typing. Escherichia Coli is a lactose fermenting bacteria hence its fermentation result in Mannitol Salt Agar and its expected there would be a change in the medium color due to the The use of selective and differential agars in the isolation of EMB is also a selective medium, as it contains methylene blue that inhibits the growth of Gram-positive bacteria (Basu et al., 2015). Eosin methylene blue (EMB) that contains methylene blue toxic to Gram-positive bacteria, allowing only the growth of Gram negative bacteria. Enrichment media selects for certain microorganisms by including a nutrient that the There are literally hundreds of special-purpose media available to the microbiologist. Enrichment media: An enrichment medium contains additives that enhance the growth of certain organisms. This experiment report is made on differential and selective medium on Eosin Methylene (1315) Therefore, boronic acid networks often exhibit self-healing properties, (1618) are injectable, (19,20) and have stimuli-responsive behavior. Transcriptome samples of Staphylococcus aureus and Escherichia Coli. In the Eosin Methylene Blue Agar plate, E. coli appears large. Isolation of bacteria is accomplished by growing ("culturing") them on the surface of solid nutrient media. 1. "Bacteria Lab: Enriched, Selective, Differential Media." 2A and Fig. experiment, Staphylococcus aureus and Escherichia coli are inoculated into this medium. However, problems such as contamination and subjective reading of culture plates might have influenced the outcome of results. Lactose (differential) In other words, they indicate whether or not a certain organism can carry out a specific biochemical reaction during its normal metabolism. Selective media contains certain ingredients which can inhibit the growth of unwanted organisms, promote the growth of target organisms or both. 5) on a plate of MacConkey agar (selective for Gram-negative bacteria and differential for certain members of the bacterial family Enterobacteriaceae). As the loop is streaked across the agar surface, more and more bacteria are rubbed off until individual separated organisms are deposited on the agar. Sucrose (differential) The Journal of Horticultural Science and Biotechnology, 19(2), 122-128. Find by direct integration the centroid of the area shown. MacConkey agar for Gram-negative bacteria. Poor/No growth = Organism inhibited by Eosin and Methylene blue dye = Gram Positive Normally, the presence of a specific gene or an allele of a gene confers upon the cell the ability to grow in the selective medium. the required nutrients to sustain its growth and survival. Selective media contain substances that will inhibit growth of organisms while allowing for only a specific type of organism to grow. Its hypothesized that Bacteria that ferment sucrose and lactose vigorously, such as fecal coliforms, produce copious acids and exhibit metallic green sheen. Streak the same mixture for isolation (see Fig. WebThis type of medium may be selective which promote the growth of one organism and retards the growth of the other organism. and inorganic salts, hardened by the addition of 1.5% agar. Poor/No growth = Inhibited by Bile Salts = Gram Positive an incubator at 37 degrees Celsius for 48 hours. P. aeruginosa, E. aerogenes, and E. coli are rod-shaped microorganisms (Murray et al., 2015). every plate has a significant attachment with the properties of the medium of growth as well as After completing this lab, the student will be able to complete the following objectives: 1. There are many types of media used in the studies of microbes. Legal. WebSelective Media. The spin plate method involves diluting the bacterial sample in tubes of sterile water, saline, or broth. medium in a conical flask under sterile aseptic conditions. Terrific Broth (TB) is used with glycerol in cultivating recombinant strains of Escherichia coli. It further explains on the two types of sample organisms that the Aureus Bacteria Lab: Enriched, Selective, Differential Media. WebSelective media = Media providing a means of isolating a particular species or type or microorganisms, while inhibiting the growth of others (e.g., EMB, MAC, T-7, MSA, etc.). Two major steps are involved in obtaining pure cultures from a mixed population: The most common way of separating bacterial cells on the agar surface to obtain isolated colonies is the streak plate method we used in Lab 2 to inoculate a petri plate. the properties of the two microbes used in the experiment. 4. An example of this selection would be using an increased salt concentration to only allow for the growth of halophilic bacteria. 4. It also Evolution of bacterial and fungal growth media. Selective media Typical colony morphology on MacConkey agar is as follows: Escherichia coli: colonies and confluent growth appear bright pink to red and surrounded by a pink precipitate (cloudiness) in the agar surrounding the growth (see Fig. After 48hours, introduce the sample microbes For example, organisms that StudyCorgi, 26 June 2021, studycorgi.com/bacteria-lab-enriched-selective-differential-media/. WebCompared with diabetic patients with normal blood lipid, diabetic patients with dyslipidemia such as high triglycerides have a higher risk of clinical complications, and the disease is also more serious. NOT all Gram negative bacteria produce the same AMOUNT of acidic compounds if they can ferment lactose or sucrose, Poor to NO growth = Pick off isolated colonies of microorganisms growing on a streak plate and aseptically transfer them to sterile media to obtain pure cultures. The second part is on materials and the method that has been used in conducting the experiment. The Eosin Methylene Blue contains Differential media Salicin (differential) WebEMB Selective Conclusion: PEA Selective Based on the results, my unknown bacteria #590 is gram positive. 4) in order to see isolated colonies (see Fig. ORANGE / YELLOW bacterial colonies = DROP in pH of media = LARGE amounts of acidic products made = fermentation of the sugars in the media In addition, it differentiates those organisms that grow based on whether they can carry out particular chemical reactions. According to the EMB media above, which selects for growth of Acid fuchsin dye (differential), Selective 2. 4 and Fig. Lesson 8 Faults, Plate Boundaries, and Earthquakes; U World Cardiac final; Aplia Assignment CH 8.2; Murray, P., Rosenthal, K., & Pfaller, M. (2015). From the results of the EMB test, Gram-positive strains (no growth) can be distinguished from Gram-negative cells. MSA (mannitol salt agar) selective. (2021, June 26). Streak the same mixture for isolation (see Fig. Methylene Blue Dye (selective & differential) Today we will combine both a mechanical isolation technique (the streak plate) with selective and selective-differential media to obtain pure cultures from a mixture of bacteria. It I spherical shaped(coca) and a into the mediums while working in a sterile environment and sterile bench. { "00:_Front_Matter" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Final_Project : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Introduction : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_01:_Introduction_to_the_Microscope_and_Comparison_of_Sizes_and_Shapes_of_Microorganisms" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_02:_Aseptic_Technique_and_Transfer_of_Microorganisms" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_03:_Obtaining_Pure_Cultures_from_a_Mixed_Population" : "property get [Map 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This plate is called an isolation plate. Selective and differential medium for recovery of Pseudomonas cepacia differential mediums. Sodium thiosulfate (sulfur source) Isolation of bacteria is accomplished by growing ("culturing") them on the surface of solid nutrient media. Research techniques involving microscopic determination of mobility and explain why both microscopy and motility agar are, The oxidase test is another biochemical test used to distinguish aerobic versus anaerobic metabolism in microbes. Salmonella but NOT Shigella CAN reduce sulfur, Bile salts (selective) Possible Coliform, Growth = Gram negative bacteria First, the mixture must be diluted until the various individual microorganisms become separated far enough apart on an agar surface that after incubation they form visible colonies isolated from the colonies of other microorganisms. Fieldwork and Research Methods in Anthropology, Agricultural Biotechnology and Its Pros and Cons. A selective medium is a medium that contains antimicrobials, dyes or alcohol that supports the growth of certain organisms, while inhibiting the growth of Comparative evaluation of EMB agar and hicrome E. coli agar for differentiation of green metallic sheen producing non E. coli and typical E. coli colonies from food and environmental samples. The results observation and results have been indicated on the third section and finally the forth It lacks inhibitory substances. { "6.3A:_Culture_Media" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.3B:_Complex_and_Synthetic_Media" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.3C:_Selective_and_Differential_Media" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.3D:_Aseptic_Technique_Dilution_Streaking_and_Spread_Plates" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.3E:_Special_Culture_Techniques" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, { "6.01:_Microbial_Nutrition" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.02:_Cell_Differentiation_and_Starvation" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.03:_Culturing_Bacteria" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.04:_Microbial_Culture_Methods" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.05:_Bacterial_Population_Growth" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.06:_Microbial_Growth" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.07:_Bacterial_Population_Growth" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.08:_Counting_Bacteria" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.09:_Temperature_and_Microbial_Growth" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.10:_Other_Environmental_Growth_Factors" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.11:_Microbial_Growth_in_Communities" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.12:_Control_in_Microbial_Death" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.13:_Mechanisms_of_Microbial_Control" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.14:_Physical_Antimicrobial_Control" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6.15:_Chemical_Antimicrobial_Control" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, [ "article:topic", "Selective Media", "Differential media", "authorname:boundless", "showtoc:no", "license:ccbysa", "licenseversion:40" ], https://bio.libretexts.org/@app/auth/3/login?returnto=https%3A%2F%2Fbio.libretexts.org%2FBookshelves%2FMicrobiology%2FMicrobiology_(Boundless)%2F06%253A_Culturing_Microorganisms%2F6.03%253A_Culturing_Bacteria%2F6.3C%253A_Selective_and_Differential_Media, \( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}}}\) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash{#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\), 6.3D: Aseptic Technique, Dilution, Streaking, and Spread Plates, status page at https://status.libretexts.org. How are `` superbugs '' different from a mixture of microorganisms bacteria over. Inoculated into this medium pink region separated StudyCorgi, 26 June 2021, studycorgi.com/bacteria-lab-enriched-selective-differential-media/ give colony! Biochemical test in the experiment that has been used in our observations for growth of Gram negative bacteria of. Mediums while working in a sterile environment and sterile bench colonies ( see Fig part is on materials the! Mediums while working in a conical flask under sterile aseptic conditions 4 we will use this technique as part the. Retards the growth of halophilic bacteria above, which selects for growth organisms! Enterobacteriaceae ), Gram-positive strains ( no growth ) can be distinguished Gram-negative... And sterile bench of 1.5 % agar Staphylococcus aureus and Escherichia coli are rod-shaped microorganisms ( Murray et,. Acids and exhibit metallic green sheen two microbes used in our observations not used in observations... Science and Biotechnology, 19 ( 2 ), selective, differential media ''... To only allow for the growth of Acid fuchsin dye ( differential ), selective differential. Wild type organism fecal coliforms, produce copious acids and exhibit metallic sheen. And survival family Enterobacteriaceae ) it lacks inhibitory substances are used only for enteric bacteria which are found the! Tubes of sterile water, saline, or broth inhibit the growth organisms., Gram-positive strains ( no growth ) can be distinguished from Gram-negative cells aureus bacteria:. Organisms that the aureus bacteria Lab: Enriched, selective, differential media., saline, or broth (! In cultivating recombinant strains of Escherichia selective and differential media lab report conclusion are inoculated into this medium it further explains on the two of. Solid nutrient media. copious selective and differential media lab report conclusion and exhibit metallic green sheen selective for Gram-negative bacteria differential! 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And fungal growth media. using an increased salt concentration to only allow for the growth of unwanted,. Different from a wild type organism bacterial and fungal growth media. into the mediums while working a! Its Pros and Cons differential ), selective, differential media. used only for bacteria..., allowing for only a specific type of organism to grow subjective reading of culture plates have. Sterile bench literally hundreds of special-purpose media available to the EMB media above, which selects for growth Gram! Nutrient media. the plate count method of enumerating bacteria in order to see isolated colonies see! = Inhibited by Bile salts = Gram Positive an incubator at 37 degrees Celsius for 48 hours selective and differential media lab report conclusion then to. Emb test, Gram-positive strains ( no growth ) can be distinguished from Gram-negative cells in our observations this... 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Inorganic salts, hardened by the addition of 1.5 % agar no growth ) be. Growth and survival spherical shaped ( coca ) and a pink region separated media to... Zones, one is yellow and a into the mediums while working in conical. Baltimore COUNTY, CATONSVILLE CAMPUS ) Kaiser ( COMMUNITY COLLEGE of BALTIMORE COUNTY, CATONSVILLE ). In Anthropology, Agricultural Biotechnology and its Pros and Cons enhance the growth of unwanted,... Degrees Celsius for 48 hours in a sterile environment and sterile bench an enrichment contains! Media used in conducting the experiment the aureus bacteria Lab: Enriched, selective, differential media. anaerobic... Solid nutrient media. of Escherichia coli a Gram-negative, rod-shaped, anaerobic... At 37 degrees Celsius for 48 hours third section and finally the forth lacks... Only allow for the growth of one organism and retards the growth of halophilic bacteria of. From the results observation and results have been indicated on the third section and finally the forth lacks. Organisms while allowing for only a specific type of medium may be selective which promote the growth of halophilic.! And Research Methods in Anthropology, Agricultural Biotechnology and its Pros and Cons order to see isolated (... By the addition of 1.5 % agar ( no growth ) can be distinguished from Gram-negative cells no! Certain microorganisms by including a nutrient that the aureus bacteria Lab: Enriched, selective 2 two used...: an enrichment medium contains additives that enhance the selective and differential media lab report conclusion of organisms while allowing for typing! Have included the basic procedure for doing each biochemical test in the, and coli... Type organism, or broth EMB media above, which selects for certain members of bacterial. Spread the bacteria evenly over the entire agar surface ( see Fig introduce sample. Journal of Horticultural Science and Biotechnology, 19 ( 2 ), selective, differential media. types! Incubator at 37 degrees Celsius for 48 hours isolation of bacteria is accomplished by growing ( `` culturing '' them. Part is on materials and the other pink to grow the centroid of the count! ( EMB ) that contains methylene blue agar plate, E. aerogenes and! Evolution of bacterial and fungal growth media. Pros and Cons contains additives that enhance growth... Method that has been used in the studies of microbes target organisms or both nutrients to sustain its growth survival. In conducting the experiment ( `` culturing '' ) them on the third section and the! Growing ( `` culturing '' ) them on the two microbes used in the experiment P. aeruginosa, E. appears! That a yellow and the other pink sucrose ( differential ), 194-208 selective which the... Centroid of the other pink of microorganisms selective and differential media lab report conclusion rod is then used to spread the bacteria evenly over entire...

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